| Title | [Application of 293 cells in in vitro detection of carcinogens] | | Author(s) | Zhao R, Zhang L, Zhong RG, Zhou L, Zeng Y | | Institution | The College of Life Science and Bio-engineering, Beijing University of Technology 100022, China. | | Source | Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2009 Feb; 23(1):47-9. | | Abstract | OBJECTIVE: To investigate the application of 293 cells to detect suspected carcinogens and provide experimental evidence by using in vitro cell transformation assay and tumorigenicity study.
METHODS: The transformation systems of cells cultured in vitro have been adopted to clarify the tumor promotive activity of Microcystin LR (MC-LR). The malignant transformation of 293 cells induced by MC-LR is tested by several methods including clone forming in soft agarose, serum requirement assay and tumor forming in mice to define the promotive activity of 293 cells.
RESULTS: 293 cell acted like tumor cells after induced by MC-LR: serum dependence decreased, anchorage independence growth in soft agarose and formed cell clones, malignant tumors appeared in SCID mice.
CONCLUSION: 293 cells were easy to culture and sensitive to environmental carcinogens so that can be used in detection of suspicious carcinogens. | | Language | chi | | Pub Type(s) | English Abstract
Journal Article
| | PubMed ID | 19799017 |
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